The isolation of ovomucoid variants differing in carbohydrate composition.

Role of carbohydrate moiety in immunoreactivity of Ovomucoid

Background An important question pertaining to glycoproteins is whether the covalently-bound carbohydrate moieties contribute to its immunogenic activity. Ovomucoid (OM) is, for these kinds of studies, a model compound in view of its relatively high carbohydrate content but the role of the carbohydrate moieties on its immunogenic activity still remains controversial. The aim of this study was t...

Ovalbumin-related gene Y protein bears carbohydrate chains of the ovomucoid type.

We have recently established the monoclonal antibodies (mAbs) specific to the major food allergen, ovomucoid, as mAb 7D, recognizing the carbohydrate moiety of ovomucoid, and mAb 6H, the peptide moiety (Biosci. Biothechnol. Biochem., 68, 2490-2497, (2004)). Using these mAbs, we found commercially available ovalbumin preparations contaminated with a considerable amount of ovomucoid together with...

The effect of different levels of dietary niacin and carbohydrate on growth performance, carcass composition and heamolymph biochemical parameters of juvenile whiteleg shrimp (Litopenaeus vannamei)

In this study, the effect of experimental diets containing different levels of carbohydrate and niacin on growth indices, carcass composition and heamolymph biochemical parameters of juvenile whiteleg shrimp (Litopenaeus vannamei) were investigated for 45 days. Shrimp with mean initial weight of 5±0.33 g were reared in 300 liter tanks with 8 treatments. Feeding was performed 4 times a day using...

The Major Allergen from Hen's Egg White: Ovomucoid (Gal d 1)

Ovomucoid (OM) is the dominant allergen from hen's egg white. Frequency of sensitization to OM varies from 34-97% in egg allergic patients. The carbohydrate moieties of OM present up to 22-29%. It consists of 186 amino acids forming three tandem domains, each providing trypsin inhibitory activity. Effective isolation and purification of OM was attained by combined IEC / SEC and by automated fra...

Isolation and carbohydrate fermentation tests of Mycoplasma agalactiae and Myeoplasma mycoides subsp.mycoides strains in Iran